Dissociation of macromolecular synthetic processes in T2-infected E. coli.

Over Expression of Biologically Active Interferon Beta Using Synthetic Gene in E. coli

In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...

Macromolecular synthesis in cells infected with Herpes simplex virus

Nutritional requirements for the reproduction of coliphage T2 in a strain of Escherichia coli.

The discovery of nutritional requirements for the reproduction of bacteriophage is not novel. Adams (1949) has clearly demonstrated a calcium requirement for T5 reproduction in Escherichia coli, strain B. Furthermore, Fowler and Cohen (1948) for T2 in B and more recently Gots and Hunt (1953) for X in irradiated E. coli, strain K12, have demonstrated the growth stimulating effect of certain amin...

Ribonucleic acid synthesis in T2-infected Escherichia coli during "stringent" control.

The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells with T2 phage stimulated uracil incorporation to a level equivalent to that of u...

Effect of prophage W on the propagation of bacteriophages T2 and T4.

Studies have been undertaken to determine whether the temperate phage omega present in Escherichia coli strain W is responsible for the inability of this strain to act as a host for T2 and T4. E. coli WS, cured of phage omega, was sensitive to T2 and T4. Lysogenation of E. coli C and WS with phage omega resulted in loss of ability to plate T2 and T4. However, E. coli K-12 lysogens still served ...